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Benzodiazepine Receptor Subunits in Avian Brain
Author(s) -
Hebebrand Johannes,
Friedl Waltraut,
Unverzagt Birgit,
Propping Peter
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00680.x
Subject(s) - photoaffinity labeling , protein subunit , cerebellum , cerebrum , gel electrophoresis , chemistry , receptor , biochemistry , biology , microbiology and biotechnology , central nervous system , neuroscience , gene
Using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and fluorography of the photoaffinity‐labeled benzodiazepine receptor in duck brain, two equally intensive bands of relative molecular masses of 53,000 and 54,000 were observed, thus being identical to the subunit pattern previously described for chicken brain. An attempt was made to chacterize further the avian subunits. Comparison of the fluorographic subunit pattern in cerebellum and telencephalon revealed a pronounced quantitative heterogeneity. Addition of ethyl β‐carboline‐3‐carboxylate resulted in the selective inhibition of the 53,000 subunit. These results are compared with the mammalian subunit pattern in the corresponding brain regions. The findings are discussed in view of the apparent contradiction between the existence of regional heterogeneity when photoaffinity labeling is applied and its lack when monoclonal antibodies are used.