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D‐1 Dopaminergic and β‐Adrenergic Stimulation of Adenylate Cyclase in a Clone Derived from the Human Astrocytoma Cell Line G‐CCM
Author(s) -
Balmforth Anthony J.,
Ball Stephen G.,
Freshney R. Ian,
Graham David I.,
McNamee H. Brian,
Vaughan Peter F. T.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00670.x
Subject(s) - cyclase , isoprenaline , endocrinology , medicine , propranolol , dopaminergic , adenylate kinase , stimulation , phentolamine , chemistry , dopamine , dopamine receptor , receptor , agonist , prolactin , biology , hormone
Clones have been isolated from the human astrocytoma cell line G‐CCM. Homogenates of clone D384 contain an adenylate cyclase that is stimulated by 3,4‐dihydroxyphenylethylamine (dopamine), noradrenaline, and isoprenaline with K a apparent values of 4, 56, and 2.7 μ M , respectively. The K a apparent value for dopamine was increased by the D‐l antagonist cis ‐flupenthixol, 25 and 100 nM, to 23 and 190 μ M , respectively, but was unaffected by propranolol (1 μf. Noradrenaline stimulation of adenylate cyclase was only partially inhibited by either propranolol (10 μ M ) or cis ‐flupenthixol (1 μ M ). Propranolol (10 μ M ), but not cis ‐flupenthixol (1 μ M ), prevented stimulation by isoprenaline. The stimulation of adenylate cyclase by dopamine and noradrenaline remained unchanged in the presence of phentolamine (1 μ M ) and sulpiride (1 μ M ). These results suggest that clone D384 contains both D‐l dopaminergic and β‐adrenergic receptors coupled to adenylate cyclase. Dopamine stimulates D384 adenylate cyclase through D‐1 receptors, isoprenaline via β‐receptors, and noradrenaline through both receptors.