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Isolation and Sequencing of a Genomic Clone for Mouse Brain Specific Small RNA
Author(s) -
Anzai Kaijiro,
Kobayashi Shunsuke,
Kitamura Narumi,
Kanai Yuri,
Nakajima Hiromichi,
Suehiro Yoshioki,
Goto Sataro
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00664.x
Subject(s) - biology , rna , nucleic acid sequence , microbiology and biotechnology , clone (java method) , genomic dna , complementary dna , intron , genetics , dna , gene
We isolated a mouse genomic clone that hybridized with small RNA present in the cytoplasm of the brain. The RNA was about 150 nucleotides long. This RNA seemed to be specific to the brain, since it was not found in the liver or kidney. The clone DNA contained a sequence homologous to 82‐nucleotide “identifier” core sequence of cDNA clones of rat. The sequence contained a split promoter for RNA polymerase III and was flanked by a 12‐nucleotide direct repeat (ATAAATAATTTA).