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Choline Uptake and Metabolism in Affinity‐Purified Cholinergic Nerve Terminals from Rat Brain
Author(s) -
Richardson Peter J.
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00646.x
Subject(s) - choline , cholinergic , acetylcholine , phosphorylation , cholinergic neuron , acetylation , choline acetyltransferase , chemistry , biochemistry , free nerve ending , endocrinology , medicine , biology , gene
Cholinergic nerve terminals were affinity purified from rat caudate nucleus. These terminals possessed both high‐( K T = 2.7 μ M ) and low‐ ( K T = 58 μ M ) affinity uptake mechanisms for exogenous [ 3 H]choline. The proportion of [ 3 H]choline acetylated was reduced from 75 to 30% under conditions of anoxia and hypoglycaemia, whereas the phosphorylation of choline increased from 4 to 52%. Choline phosphorylation was also increased when the terminals were preloaded with choline. The affinity‐purified terminals were shown to release acetylcholine in a Ca 2+ ‐dependent manner on depolarization. The relationship between choline acetylation and phosphorylation in the cholinergic nerve terminal is discussed.