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Effects of Hypothermia on the In Vivo Measurement of Rapid Axonal Transport in the Rat: A Cautionary Note
Author(s) -
Padilla Stephanie,
Lyerly Donald
Publication year - 1986
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1986.tb00642.x
Subject(s) - axoplasmic transport , hypothermia , in vivo , superior colliculus , fucosylation , biology , chemistry , neuroscience , fucose , glycoprotein , biochemistry , physiology , microbiology and biotechnology
Rapid axonal transport of glycoproteins was examined in the retinofugal projections of hypothermic and normothermic adult male Long‐Evans hooded rats previously receiving intraocular injections of [ 3 H]fucose. The amount of retinal fucosylation appeared normal in the hypothermic animals 3.5 h after isotope injection, but glycoprotein transport was reduced relative to normothermic controls. This reduction was especially pronounced in the most distal structure of the retinofugal tract (superior colliculus). We conclude that rapid axonal transport decreases with reductions in mammalian body temperature. This finding emphasizes the importance of controlling body temperature in in vivo studies of mammalian axonal transport.