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Purification of Astroprotein (Astrocyte‐Specific Cerebroprotein) by Reversed‐Phase C‐1 HPLC
Author(s) -
Morimoto Kazuyoshi,
Hashimoto Tadao,
Maeda Yasutaka,
Hayakawa Toru,
Tagawa Kunio,
Mogami Heitaro
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb12915.x
Subject(s) - trifluoroacetic acid , chromatography , high performance liquid chromatography , chemistry , acetonitrile , sodium dodecyl sulfate , phase (matter) , sodium , reversed phase chromatography , organic chemistry
A method for purification of astroprotein (an astrocyte‐specific cerebroprotein) with HPLC is described. A linear gradient from 30 to 70% acetonitrile in 0.1% trifluoroacetic acid (pH 2.2) was applied to the re‐versed‐phase C‐l (particle size 10 μm) column. Cerebroproteins from the crude extract from human glioma were clearly separated by this procedure. Highly purified astroprotein was homogeneous by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and has immunoreactivity to antiserum against astroprotein. Reversed‐phase C‐1 HPLC offers advantages over previously available preparative techniques in the higher purity and better separation time of the products.