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Effect of Electrical Stimulation on Phosphoinositide Metabolism in Rat Sciatic Nerve In Vivo
Author(s) -
Goswami S. K.,
Gould R. M.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb12908.x
Subject(s) - inositol , stimulation , inositol phosphate , phosphatidylinositol , in vivo , endocrinology , medicine , metabolism , chemistry , phosphate , sciatic nerve , biology , biochemistry , receptor , phosphorylation , microbiology and biotechnology
The metabolism of phosphoinositides in rat sciatic nerves in vivo during electrical stimulation was studied. Nerves were prelabeled by injection of [2‐ 3 H]‐ myo ‐inositol alone for periods of 2 and 20 h or together with [ 32 P]orthophosphate for 2 h and then electrically stimulated (100 Hz) for 5 or 20 min. Contralateral unstimulated nerve served as the control. When tritiated myo ‐inositol was used alone for prelabeling the nerves, approximately 6% and 14% of the label was incorporated into lipids after 2 h and 20 h, respectively. Both 5 and 20 min of electrical stimulation caused an insignificant change in the percentage of radioactivity recovered in lipids from the nerves prelabeled with either myo ‐inositol or with a mixture of myo ‐inositol and phosphate. The proportion of label associated with phosphoinositides of nerves prelabeled with myo ‐inositol for both 2 h and 20 h showed an increase in phosphatidyl‐inositol‐4‐phosphate at the expense of phosphatidylinositol in stimulated nerves. Similar results were obtained with nerves prelabeled for 2 h with a mixture of [ 32 P]orthophosphate and [2‐ 3 H] myo ‐inositol. No significant changes in the radioactivity associated with water‐soluble inositol phosphates were found in stimulated versus control nerves.