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Glycosaminoglycans of Rat Cerebellum: II. A Developmental Study
Author(s) -
Werz W.,
Fischer G.,
Schachner M.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb12902.x
Subject(s) - keratan sulfate , glycosaminoglycan , dermatan sulfate , heparan sulfate , hyaluronic acid , glucosamine , chondroitin sulfate , cerebellum , chondroitin , sulfate , chemistry , biochemistry , sulfation , endocrinology , medicine , biology , anatomy , organic chemistry
Total and individual glycosaminoglycans (GAGs) were determined in rat cerebellum in tissue explants at various postnatal ages. The major constituents of GAGs were chondroitin sulfate (CS), hyaluronic acid (HA), and heparan sulfate (HS). Dermatan sulfate (DS) and keratan sulfate (KS) could not be detected and therefore each amounts to <5% of all GAGs at all ages studied. HA was the prominent GAG during postnatal development and only a minor constituent at adult ages, whereas CS was the predominant GAG in adulthood. HS remained relatively constant throughout development. The incorporation of [ 3 H]glucosamine into individual GAGs was highest for HS at postnatal day 6, whereas HA showed intermediate and CS the lowest levels of incorporation during the first postnatal week. All major GAGs showed the lowest incorporation values at adult ages.