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Expression of A and B Types of Monoamine Oxidase in Differentiated Neuroblastoma Hybrid Cells
Author(s) -
Nakano T.,
Nagatsu T.,
Higashida H.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb12879.x
Subject(s) - neuroblastoma , monoamine oxidase , monoamine oxidase b , cell type , intracellular , cell culture , chemistry , endocrinology , medicine , microbiology and biotechnology , biology , enzyme , cell , biochemistry , genetics
The total activities of monoamine oxidase (MAO) and the ratio of type B/type A activities were determined in mouse neuroblastoma N1E‐115 cells, and in NX31T and NG108‐15 hybrid cells derived from mouse neuroblastoma × rat sympathetic ganglion hybrid or mouse neuroblastoma × rat glioma hybrid cells. N1E‐115 and NX31T cells possessed type A activities exclusively, although NG108‐15 cells showed both type A (65–90%) and type B (10–35%) MAO activities. The activity of type A MAO in NX31T and N1E‐115 cells was relatively constant during culturing periods in the presence or absence of dibutyryl cyclic AMP (Bt 2 cAMP), whereas total MAO activity and the ratio of type B MAO/type A MAO in NG108‐15 cells increased as a function of culture periods. Prostaglandin E 1 (PGE 1 ) and theophylline, the best known combination to increase intracellular cyclic AMP content of NG108‐15 cells, caused similar increases of MAO and of the type B/type A ratio in NG108‐15 cells. The results suggest that MAO activity and expression of MAO B activity are regulated in NG108‐15 cells in a cyclic AMP‐dependent manner.