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Measurement of Substance P Metabolites in Rat CNS
Author(s) -
Sakurada T.,
Grevés P.,
Stewart J.,
Terenius L.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb12874.x
Subject(s) - spinal cord , chromatography , chemistry , radioimmunoassay , agarose , agarose gel electrophoresis , microbiology and biotechnology , biochemistry , biology , dna , neuroscience
A procedure based on ion‐exchange chromatography for chemical separation and radioimmunoassays for quantitation of substance P(SP), the SP(1–7), and C‐terminal fragments, respectively, has been developed. The procedure allows the determination of these fragments in the presence of large (i.e., 50‐ to 100‐fold) excess of parent compound. The chemical identity of isolated SP and fragments was studied with preparative electrophoresis on dilute agarose gel and with HPLC. The activity identified as SP(1–7) comigrated with the authentic standard whereas practically all activity isolated as C‐terminal fragments comigrated with SP(5–11). The levels of C‐terminal fragments in rat brain areas rich in SP and in spinal cord were 1–2% of those of parent compound. The levels of SP(1–7) were always higher, in the spinal cord markedly higher (three to five times). Postmortem storage of samples from brain and spinal cord indicated that SP(1–7) levels fell more rapidly than those of SP or C‐terminal fragments.