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Deprenyl Protects Dopamine Neurons from the Neurotoxic Effect of 1‐Methyl‐4‐Phenylpyridinium Ion
Author(s) -
Mytilineou Catherine,
Cohen Gerald
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb10556.x
Subject(s) - mptp , dopamine , monoamine oxidase , chemistry , neurotoxin , toxicity , monoamine neurotransmitter , pharmacology , biochemistry , biology , endocrinology , enzyme , dopaminergic , serotonin , receptor , organic chemistry
1‐Methyl‐4‐phenylpyridinium ion (MPP + ) is the product of the metabolic oxidation of 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP) by mono‐amine oxidase (MAO). MPP + is toxic to 3,4‐dihydroxy‐phenylethylamine (dopamine, DA) neurons in explant cultures of rat embryonic midbrain. Addition of 2.5 μ M MPP + to the feeding medium for 6 days results in significant reduction of the DA levels in the cultures (to 19% of control) as well as in the uptake of [ 3 H]DA (to 32% of control). When the cultures are treated with the MAO inhibitor deprenyl (10 μ M ) 24 h prior to and during exposure to MPP + , the DA neurons are protected from the toxicity of the drug. In the combined deprenyl plus MPP + treatment, the levels of DA in the cultures remain at the control range and the [ 3 H]DA uptake is reduced to only 73% of control. These results indicate that MAO is involved in the toxicity of MPP + on DA neurons.