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Neurotransmitter Systems in Morphologically Undifferentiated Human Y‐79 Retinoblastoma Cells: Studies of GABAergic, Glycinergic, and β‐Adrenergic Systems
Author(s) -
Madtes Paul,
Kyritsis Athanassios,
Chader Gerald J.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb10541.x
Subject(s) - glycine receptor , neurotransmitter , gabaergic , glycine , antagonist , retinoblastoma , receptor , biology , neurotransmitter receptor , microbiology and biotechnology , endocrinology , medicine , chemistry , biochemistry , amino acid , gene
Elements of three neurotransmitter systems were investigated in morphologically undifferentiated human Y‐79 retinoblastoma cells in suspension culture. Specific γ‐aminobutyric acid (GABA) uptake, GABA binding, and glycine binding were absent from these cells, although the cells had been shown to exhibit an active uptake and release of [ 3 H]glycine. Binding and competition studies using both α‐and β‐adrenergic ligands indicated the presence of a β‐adrenergic receptor. This finding was confirmed by treatment of the cells with β‐agonists in competition with a β‐antagonist and with an α‐antagonist; the level of cyclic AMP was competitively stimulated. Therefore, human Y‐79 cells in suspension culture contain β‐adrenergic receptors, and not glycinergic or GABAergic ones. Thus, the Y‐79 cells may be of use in studying the factors involved in developmental regulation of neurotransmitter function.