Premium
Synthesis of γ‐Glutamyldopamine and Other Peptidoamines in the Nervous System of Aplysia californica
Author(s) -
McCaman Marilyn W.,
Stetzler Judy,
Clark Brian
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb10540.x
Subject(s) - aplysia , tryptamine , octopamine (neurotransmitter) , biogenic amine , tyramine , dopamine , amine gas treating , conjugate , chemistry , biochemistry , hydrolysis , chromatography , high performance liquid chromatography , biology , neurotransmitter , organic chemistry , serotonin , endocrinology , mathematical analysis , receptor , mathematics , evolutionary biology
The synthesis of a series of γ‐glutamyl amines (γ‐Glu‐amines), including γ‐Glu‐dopamine, γ‐Glu‐5‐hydroxytryptamine, γ‐Glu‐octopamine, γ‐Glu‐tryptamine, γ‐Glu‐tyramine, and γ‐Glu‐phenylethylamine, by nervous tissue of the marine mollusc Aplysia californica is described. After ganglia were incubated in vitro with 14 C‐amines, the unchanged amine and a new 14 C‐labeled product, identified as the γ‐Glu conjugate of the amine, were isolated from the tissue extracts. Identification was made by comparing the chromatographic properties (HPLC, TLC, and LC) of the isolated conjugates with chemically synthesized γ‐Glu‐amines before and after acid hydrolysis.