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Serotonin Inhibits Acetylcholine Release from Rat Striatum Slices: Evidence for a Presynaptic Receptor‐Mediated Effect
Author(s) -
Gillet G.,
Ammor S.,
Fillion G.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb10523.x
Subject(s) - methysergide , acetylcholine , serotonergic , chemistry , serotonin , cholinergic , 5 ht receptor , striatum , medicine , endocrinology , tetrodotoxin , pharmacology , receptor , dopamine , biology , biochemistry
Rat brain striatum slices were incubated with [ 3 H]choline, perfused with a physiological buffer, and stimulated by perfusion with a K + ‐enriched buffer for 2 min. The tritium overflow evoked by K + was decreased by 5‐hydroxytrytamine (serotonin, 5‐HT) (maximal inhibition 10 –6 M ). This effect of 5‐HT was mimicked by several agonists (5‐methoxytryptamine, N,N ‐dimethyltryptamine, bufotenin) and blocked by serotonergic antagonists (methiothepin, methysergide, cinanserin) but not by haloperidol; methiothepin and methysergide alone slightly increased the K + ‐evoked overflow of tritium ( 3 H). Inhibition of the tritium release by 5‐HT was not suppressed in the presence of tetrodotoxin (TTX) (10 –6 M ) These results suggest that 5‐HT tonically inhibits acetylcholine (ACh) release from striatal cholinergic neurons by acting on a presynaptic receptor localized on cholinergic terminals.