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Role of Hyperthermia in Effects of Electroconvulsive Shock on Protein Synthesis in the Rabbit
Author(s) -
Nowak Thaddeus S.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08762.x
Subject(s) - hyperthermia , electroconvulsive shock , in vitro , protein biosynthesis , heat shock protein , shock (circulatory) , endocrinology , medicine , chemistry , biology , pharmacology , biochemistry , gene
The effects of electroconvulsive shock (ECS) on rectal temperature (T R ) and on protein synthesis in brain and liver were compared in rabbit, rat, and mouse. Protein synthesis status was assessed using an in vitro amino acid incorporation method which provides information equivalent to polyribosome profiles. In the rabbit, T R rose from 39.5 ± 0.4°C to 40.4 ± 0.2°C within 10 min following a single ECS, and significant hyperthermia persisted for at least 60 min. This effect was markedly attenuated in animals housed at 4°C. In vitro protein synthesis activities of rabbit brain and liver preparations were significantly reduced following ECS only in those animals whose T R exceeded 40°C. In the rat, ECS gave rise to a significant hyperthermia, but in no case did T R exceed 40°C, and protein synthesis activity of brain supernatants was not affected. In the mouse, ECS reduced T R and had no effect on in vitro protein synthesis activity. These results demonstrate that the unique sensitivity of protein synthesis in rabbit tissues to electroconvulsive shock is a direct consequence of the hyperthermia that arises following ECS in this species.