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Effects of Rat Ovariectomy on CSF Monoamine Metabolite Levels and Elimination
Author(s) -
Duval Christine,
Mignot Emmanuel
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08759.x
Subject(s) - metabolite , monoamine neurotransmitter , endocrinology , chemistry , medicine , pharmacology , biology , serotonin , receptor
Cerebrospinal fluid (CSF) was removed from the third ventricle of anesthetized male, female, and ovariectomized rats. CSF 3,4‐dihydroxyphenylethylamine and serotonin metabolite levels [dihydroxyphenylacetic acid, homovanillic acid (HVA), and 5‐hydroxyindoleacetic acid (5‐HIAA)] were determined on 15‐min samples by liquid chromatography coupled with electrochemical detection. Monoamine oxidase inhibition was used for studying metabolite turnover in the CSF. No difference was observed between male, ovariectomized, and sham‐operated female rats. However, ventricular CSF HVA and 5‐HIAA levels were significantly higher in the ovariectomized than in the sham‐operated rats. These differences do not reflect effects of ovariectomy on brain metabolite production but indicate slower metabolite elimination from the CSF.