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Ganglioside GM 1 Does Not Initiate, but Enhances Neurite Regeneration of Nerve Growth Factor‐Dependent Sensory Neurones
Author(s) -
Doherty Patrick,
Dickson John G.,
Flanigan Thomas P.,
Walsh Frank S.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08752.x
Subject(s) - neurite , nerve growth factor , neurofilament , ganglioside , dorsal root ganglion , regeneration (biology) , microbiology and biotechnology , in vitro , axon , biology , sensory system , chemistry , neuroscience , biochemistry , immunology , immunohistochemistry , receptor
An enzyme‐linked immunoadsorbent assay (ELISA) for neurofilament protein was utilised to quantify the effect of exogenous ganglioside on neurite regeneration in cultures of dorsal root ganglion neurones. In contrast to nerve growth factor (NGF), ganglioside GM 1 (100 μg/ml) failed to support neuronal survival and neurite regeneration as quantified by the ELISA assay and confirmed by morphological criteria. However, the simultaneous presence of GM 1 (100 μg/ml) and NGF (0.5–5 ng/ml) throughout a 5‐day period of culture resulted in an enhancement of previously reported NGF‐induced increases in the expression of neurofilament protein. Further, the addition of GM 1 (0–200 μg/ml) at 48 h in vitro to cultures initially established in the presence of 5 ng/ml NGF substantially increased the subsequent expression of neurofilament protein, this response being both independent of and not potentiated by NGF. The results in the present system suggest that GM 1 cannot initiate a programme of neurite regeneration; however, GM 1 can enhance this process with the response being secondary to the effect of NGF.