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Selective Neurotoxic Lesions of Descending Serotonergic and Noradrenergic Pathways in the Rat
Author(s) -
Berge OddGeir,
Fasmer Ole Bernt,
Tveiten Lars,
Hole Kjell
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08738.x
Subject(s) - serotonergic , 5,7 dihydroxytryptamine , spinal cord , monoaminergic , neurotoxin , medicine , endocrinology , serotonin , monoamine neurotransmitter , lumbar , saline , lumbar spinal cord , norepinephrine , anesthesia , chemistry , central nervous system , dopamine , anatomy , receptor , psychiatry
Abstract: The ability of neurotoxic substances to induce selective lesions of the descending monoaminergic pathways in rats was investigated. Saline, 6‐hydroxydopamine, 5,6‐dihydroxytryptamine, or 5,7‐dihydroxytryp tamine were administered into the lumbar subarachnoid space through a chronically indwelling catheter. The lesions were evaluated 2–3 weeks later by in vitro uptake of [ 3 H]noradrenaline and [ 14 C]5‐hydroxytryptamine into synaptosomal preparations from the frontal cortex, brain stem, cervical spinal cord, and lumbar spinal cord of each animal. There was no difference in uptake between saline‐injected and noncatheterized controls and no significant changes in cortcial uptake after any of the treatments (dose range of neurotoxins: 0.6–80 μg). In the lumbar spinal cord, 6‐hydroxydopamine (5–80 μg) reduced the [ 3 H]noradrenaline uptake by approximately 90% with no effects on [ 14 C]5‐hydroxytryptamine uptake, whereas 5,6‐dihydroxytryptamine reduced the uptake of [ 14 C]5‐hydroxytryptamine by 90% (20–80 μg). [ 3 H]Noradrenaline uptake was unaffected by lower doses of 5,6‐dihydroxy tryptamine but fell by 45–55% after 40–80 μg. 5,7‐Dihydroxytryptamine (10–80 μg) reduced [ 3 H]noradrenaline uptake by 90–95% and [ 14 C]5‐hydroxytryptamine uptake by approximately 80% (5–80 μg) in the lumbar cord. It is concluded that intrathecal administration of suitable doses of neurotoxins may produce extensive selective lesions of descending noradrenergic and serotonergic path ways.