Premium
Assay of Brain Calmodulin Levels Using High‐Performance Liquid Chromatography
Author(s) -
Loullis Costas C.,
Antonian Lida,
Rauh Charles E.,
Fliegner Karsten,
Coupet Joseph,
Lippa Arnold S.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08732.x
Subject(s) - calmodulin , radioimmunoassay , high performance liquid chromatography , chromatography , hypothalamus , brain tissue , chemistry , biochemistry , biology , endocrinology , neuroscience , enzyme
A simple, sensitive, and efficient HPLC method for the determination of calmodulin levels in brain tissue extracts is described. The assay is linear with respect to both calmodulin and protein concentrations. The specificity and validity of this assay for calmodulin is demonstrated by parallel radioimmunoassay determinations which give equivalent results. Determination of calmodulin levels in various brain regions revealed a high concentration of this protein in the hypothalamus, by comparison to other areas examined.