Large‐Scale Purification of  Torpedo  Electric Organ Synaptosomes | Zendy

Israël M. | Zendy; Lazereg S. | Zendy; Lesbats B. | Zendy; Manaranche R. | Zendy; Morel N. | Zendy

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Large‐Scale Purification of Torpedo Electric Organ Synaptosomes

Author(s) -

Israël M.,

Lazereg S.,

Lesbats B.,

Manaranche R.,

Morel N.

Publication year - 1985

Publication title -

journal of neurochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.75

H-Index - 229

eISSN - 1471-4159

pISSN - 0022-3042

DOI - 10.1111/j.1471-4159.1985.tb08731.x

Subject(s) - electric organ , torpedo , depolarization , acetylcholine , chemistry , chromatography , fraction (chemistry) , calcium , membrane , biophysics , biochemistry , biology , acetylcholine receptor , endocrinology , organic chemistry , receptor

A procedure for the large‐scale purification of Torpedo electric organ synaptosomes is described. The synaptosomal fraction obtained is very pure as judged from biochemical and morphological data. In addition, acetylcholine (ACh) release was demonstrated after KCl depolarization of synaptosomes in the presence of calcium. Two hundred grams of electric organ can be fractionated in a single run, allowing biochemical studies on presynaptic membrane constituents.

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