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Effects of Aging and Morphine Administration on Calmodulin and Calmodulin‐Regulated Enzymes in Striata of Mice
Author(s) -
Hoskins B.,
Ho I. K.,
Meydrech E. F.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb08726.x
Subject(s) - calmodulin , medicine , endocrinology , adenylate kinase , phosphodiesterase , cyclase , morphine , chemistry , saline , enzyme , cyclic nucleotide phosphodiesterase , radioimmunoassay , biology , calcium , stimulation , biochemistry
Male ICR mice, young (25‐days old), mature (3‐months old), and old (22 months), were injected with morphine sulfate (10 mg/kg, s.c.) or were implanted with morphine pellets (75 mg). Controls received saline injections or placebo pellets. One hour after injections and 72 h after pellet implantations, the mice were decapitated and striatal regions were removed for the following analyses: calmodulin (CaM) levels via radioimmunoassay and activities of cyclic nucleotide phosphodiesterases, adenylate and guanylate cyclases, and Ca 2+ , Mg 2+ ‐ATPase. Acute morphine treatment produced the following: (1) increases in calmodulin levels in the young and old mice while having no effect on mature levels; (2) increases in activities of guanylate cyclase of mature mice while decreasing those of the old mice; (3) no effects on activity of adenylate cyclase; (4) decreased activity of cyclic AMP‐phosphodiesterase in young mice only; (5) decreased activity of Ca 2+ , Mg 2+ ‐ATPase in the old mice only. The only changes found in striata from morphinetolerant mice when compared with age‐matched controls were elevations in cyclic GMP‐phosphodiesterase activities in all three age groups. Differences in control values of the three age groups were as follows: CaM levels, mature > old > young; Ca 2+ , Mg 2+ ‐ATPase activity, old > mature‐young. The results indicate age‐induced changes in cellular regulation and biochemical responses to morphine.