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Glucose Transport in Astrocytes: Regulation by Thyroid Hormone
Author(s) -
Roeder Lois M.,
Williams Irene B.,
Tildon J. Tyson
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07239.x
Subject(s) - triiodothyronine , medicine , endocrinology , thyroid , hormone , incubation , astrocyte , deoxyglucose , thyroid hormones , hexose , substrate (aquarium) , biology , chemistry , central nervous system , biochemistry , enzyme , ecology
Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate‐saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent K m of approximately 1 m M. Maintenance of the cells in medium containing thyroid‐hormone‐free serum for 3, 6, or 9 days resulted in significantly reduced rates of hexose transport. Addition of exogenous triiodothyronine to the transport incubation medium of these “hypothyroid′’ cells markedly increased the net rate of 2‐deoxyglucose uptake within 60 s to values equal to or above those of control cultures (cells maintained in normal serum). These findings support a key role for thyroid hormone in the transport of glucose across plasma membranes of brain cells and demonstrate the presence of this regulatory system in astrocytes.