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Synaptosomal Phospholipase D Potential Role in Providing Choline for Acetylcholine Synthesis
Author(s) -
Hattori Hiroshi,
Kanfer Julian N.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07229.x
Subject(s) - acetylcholine , choline , synaptosome , acetylcholinesterase , phosphatidylcholine , chemistry , biochemistry , phospholipase , phospholipase d , choline acetyltransferase , enzyme , sodium , biology , endocrinology , phospholipid , membrane , organic chemistry
The phospholipase D of the rat brain synaptic membrane possesses the highest activity of this enzyme of any mammalian tissue examined. The synaptic phospholipase D activity is latent and barely detectable in the absence of 4 m M sodium oleate. Several other fatty acids were either less effective or ineffective as stimulators of activity compared to this monounsaturated fatty acid. The activity was decreased by hemicholinium‐3, an inhibitor of choline uptake and slightly activated by neostigmine, an acetylcholinesterase inhibitor. Incubation of synaptosomes in the presence of sodium oleate and acetyl‐coenzyme A resulted in the formation of a product chromatographing with acetylcholine. Acetylcholine formation was nearly undetectable in the absence of sodium oleate or acetyl‐coenzyme A. These results implicate synaptosomal phospholipase D in releasing choline from phosphatidylcholine for acetylcholine formation.