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Activation of Muscarinic and of α 1 ‐Adrenergic Receptors on Astrocytes Results in the Accumulation of Inositol Phosphates
Author(s) -
Pearce Brian,
CambrayDeakin Martin,
Morrow Christine,
Grimble Jill,
Murphy Sean
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07224.x
Subject(s) - carbachol , inositol , inositol phosphate , muscarinic acetylcholine receptor , endocrinology , medicine , intracellular , receptor , astrocyte , egta , cholinergic , biology , chemistry , inositol trisphosphate , biochemistry , calcium , central nervous system
Astrocyte‐enriched cultures prepared from the newborn rat cortex incorporated [ 3 H] myo ‐inositol into intracellular free inositol and inositol lipid pools. Noradrenaline and carbachol stimulated the turnover of these pools resulting in an increased accumulation of intracellular [ 3 H]inositol phosphates. The effects of noradrenaline and carbachol were dose‐dependent and blocked by specific α 1 ‐adrenergic and muscarinic cholinergic receptor antagonists, respectively. The increase in [ 3 H]inositol phosphate accumulation caused by these receptor antagonists was virtually unchanged when cultures were incubated in Ca 2+ ‐free medium, but was abolished when EGTA was also present in the Ca 2+ ‐free medium. Cultures of meningeal fibroblasts, the major cell type contaminating the astrocyte cultures, also accumulated [ 3 H] myo ‐inositol, but no increased accumulation of [ 3 H]inositol phosphates was found in response to either noradrenaline or carbachol.