Premium
Differential Effects of Lithium on Muscarinic Receptor Stimulation of Inositol Phosphates in Rat Cerebral Cortex Slices
Author(s) -
Batty Ian,
Nahorski Stefan R.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07221.x
Subject(s) - inositol , carbachol , inositol phosphate , endocrinology , medicine , agonist , muscarinic acetylcholine receptor , chemistry , inositol trisphosphate receptor , muscarinic agonist , phospholipase c , inositol trisphosphate , lithium (medication) , stimulation , receptor , biology , biochemistry
The accumulation of labelled inositol mono‐, bis‐, and trisphosphate in rat cerebral cortex slices was examined following preincubation with [ 3 H]inositol. The muscarinic receptor agonist carbachol produced a rapid and sustained increased accumulation of each labelled inositol phosphate both in the presence and absence of 5 m M lithium. Lithium potentiated carbachol‐stimulated accumulation of inositol monophosphate (EC 50 0.5 m M ) and inositol bisphosphate (EC 50 4 m M ) in a concentration‐dependent manner. However, exposure to lithium in the presence of the muscarinic agonist produced a concentration‐ and time‐dependent inhibition of inositol trisphosphate accumulation that was not related to receptor desensitisation. Although the present data do suggest that polyphosphoinositides are substrates for agonist‐stimulated phospholipase C in brain, these results may not be entirely consistent with the production of inositol mono‐ and bisphosphate through inositol trisphosphate dephosphorylation. Furthermore, these data suggest site(s) additional to inositol monophosphatase that are affected by lithium.