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Uptake and Anterograde Axonal Transport of Aleuria Lectin in Retinal Ganglion Cells of the Rabbit
Author(s) -
Ohlson C.,
Nilsson E.,
Karlsson J.O.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07169.x
Subject(s) - lectin , axoplasmic transport , axon , ganglion , endocytosis , chemistry , biophysics , microbiology and biotechnology , geniculate ganglion , retinal , biology , anatomy , neuroscience , biochemistry , pathology , medicine , cell , alternative medicine , palsy
A fucose‐specific lectin from Aleuria aurantia was used to study the dynamics of neuronal membrane glycoproteins. Albino rabbits received vitreal injections of affinity‐purified 125 I‐ Aleuria lectin. The radioactive probe was internalized by adsorptive endocytosis into retinal ganglion cells, and transported intact down to the nerve terminals in the contralateral geniculate bodies and superior colliculi. We found that the radiolabeled lectin was transported with at least two distinct rates (I, $205 mm/day; II, $45 mm/day) corresponding to the two rapid phases of anterograde transport of endogenous polypeptides described earlier in this system. This is the first evidence that an exogenous macromolecule may be transported along the axon at more than one velocity.