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Reversible Dihydropyridine Isothiocyanate Binding to Brain Calcium Channels
Author(s) -
Greenberg David A.,
Cooper Edward C.,
Carpenter Celia L.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb07148.x
Subject(s) - nitrendipine , dihydropyridine , chemistry , binding site , isothiocyanate , calcium , voltage dependent calcium channel , biophysics , calcium channel , isradipine , ileum , biochemistry , biology , organic chemistry
Voltage‐dependent calcium channels from ileal smooth muscle can be affinity‐labeled with a [ 3 H]dihydropyridine isothiocyanate radioligand. We examined the binding of this agent to brain membranes, to compare the properties of calcium channel drug binding sites in brain with those previously described in ileum. In brain, the [ 3 H]dihydropyridine isothiocyanate labels sites that correspond in number and pharmacologic characteristics to binding sites for the classic calcium entry blocker, [ 3 H]nitrendipine. However, in contrast to the covalent nature of dihydropyridine isothiocyanate binding in ileum, brain calcium channels are labeled reversibly. This difference in binding properties may reflect structural variations in voltage‐dependent calcium channels in different tissues.