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Presence and Ontogeny of Enkephalin and Substance P in the Chick Ciliary Ganglion
Author(s) -
White Jeffrey D.,
Krause James E.,
Karten Harvey J.,
McKelvy Jeffrey F.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05562.x
Subject(s) - ontogeny , ciliary ganglion , ganglion , substance p , enkephalin , biology , neuroscience , endocrinology , medicine , anatomy , neuropeptide , opioid , biochemistry , receptor
The avian ciliary ganglion has been reported to contain both enkephalin and substance P in preganglionic terminals. However, extensive biochemical characterization of these antigens has not been completed. Using radioimmunoassays specific for Met 5 ‐and for Leu 5 ‐enkephalin and for substance P we identified immunoreactive substances in ganglionic extracts that comigrate on HPLC columns with standard Met 5 ‐ and Leu 5 ‐enkephalin and with substance P. The ontogeny of Met 5 ‐enkephalin and substance P during embryogenesis was determined in ganglionic extracts and we found that the content of Met 5 ‐enkephalin in the ganglion reached a peak at embryonic stage 37 whereas the content of substance P in the ganglion reached its maximum in the adult.