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Developmental Expression of the Myelin‐Associated Glycoprotein in the Peripheral Nervous System Is Different from That in the Central Nervous System
Author(s) -
Frail Donald E.,
Webster Henry de F.,
Braun Peter E.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05559.x
Subject(s) - central nervous system , peripheral nervous system , myelin , nervous system , glycoprotein , myelin associated glycoprotein , messenger rna , reticulocyte , peripheral , biology , in vitro , neuroscience , sciatic nerve , microbiology and biotechnology , anatomy , medicine , biochemistry , gene
We recently characterized two developmentally regulated myelin‐associated glycoprotein (MAG) polypeptides synthesized by mouse brain mRNA in vitro. We now extended these studies to include the peripheral nervous system (PNS). Total cytoplasmic RNA was isolated from the sciatic nerves of 7‐, 12‐, and 17‐day‐old and adult rats and translated in vitro in a rabbit reticulocyte lysate system. In contrast to results in the CNS, it appears that only one MAG polypeptide, p67MAG, is synthesized by PNS mRNA at all ages. The implications of these findings are discussed with respect to recent observations concerning both the localization of MAG and the synthesis of MAG in the PNS of dysmyelinating mutant mice.