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Differences in L‐[ 3 H]Glutamate Accumulation and Endogenous L‐Glutamate Content in Synaptic Vesicles from Mice Selectively Bred for Differences in Ethanol Sensitivity
Author(s) -
Disbrow Jeffrey K.,
Ruth James A.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05556.x
Subject(s) - glutamate receptor , endogeny , ethanol , synaptic vesicle , chemistry , neuroscience , biochemistry , biology , vesicle , membrane , receptor
The ATP‐stimulated accumulation of L‐[ 3 H]glutamate by whole brain synaptic vesicle preparations from long‐sleep and short‐sleep mice, lines selectively bred for difference in sleep time response to acute ethanol administration, was examined. L‐[ 3 H]Glutamate accumulation in vesicles from short‐sleep mice was approximately twice that observed in vesicles from longsleep mice at three glutamate loading concentrations. The vesicular content of endogenous L‐glutamate in crude and enriched vesicle preparations from short‐sleep mice was approximately 1.5‐fold higher than in vesicles from long‐sleep mice. The data suggest that L‐glutamate associated with synaptic vesicles may serve a role in glutamate neurosecretion.