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Denervation Supersensitivity of the Rat Pineal to Norepinephrine‐Stimulated [ 3 H]Inositide Turnover Revealed by Lithium and a Convenient Procedure
Author(s) -
Zatz Martin
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05479.x
Subject(s) - chemistry , inositol , chromatography , stimulation , lithium (medication) , sephadex , pineal gland , biochemistry , endocrinology , receptor , biology , enzyme , circadian rhythm
A convenient procedure for the assay of myo‐ [2– 3 H(N)]inositol ([ 3 H]inositol) metabolites in cells or small amounts of tissue was developed. The procedure is a composite of modifications of published methods. After preincubation with [ 3 H]inositol, rat pineal glands were disrupted in an acidified organic solvent mixture. Lipids were separated from the hydrophilic products and precursor using Sephadex G‐25 columns and further analyzed by TLC. Hydrophilic products were further analyzed by anion‐exchange column chromatography using Dowex AG1‐X8 (formate form). In the presence of lithium, increases in inositol phosphates consequent to stimulation of the glands by norepinephrine were apparent within 10 min. The response in denervated glands was considerably greater than in intact pineals.