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Phospholipid Methylase Activity, [ 3 H] S ‐Adenosyl‐l‐Homocysteine Binding, and S ‐Adenosyl‐l‐Methionine and S ‐Adenosyl‐l‐Homocysteine Levels in Rat Brain During Maturation
Author(s) -
Gharib A.,
Rey C.,
Fonlupt P.,
Sarda N.,
Pacheco H.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05470.x
Subject(s) - phospholipid , methyltransferase , homocysteine , methylation , methionine , biochemistry , endogeny , endocrinology , medicine , chemistry , biology , membrane , amino acid , gene
The changes in activity of phospholipid methyltransferase I and [ 3 H] S ‐adenosyl‐l‐homocysteine ([ 3 H]SAH) binding were determined in cortical membrane preparations from newborn rats and rats 1, 2, and 8 months old. The activity of phospholipid methyltransferase I and the [ 3 H]SAH binding were significantly greater (respectively, +30 and +40%) in newborn rats than in 1‐, 2‐, and 8‐month‐old rats. The methylated products at days 1 and 30 were identical. These changes in methyltransferase activity may be correlated with variations in concentration of S ‐adenosyl‐l‐methionine (SAM) and SAH. The endogenous SAM level was higher and the SAH level was lower in newborn compared with adult rats. These data suggested that the processes of methylation were favored in newborn rats. The modifications observed after treatment with l‐homocysteine reinforced this hypothesis.