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Tetraenoic Species Are Conserved in Muscarinically Enhanced Inositide Turnover
Author(s) -
Rooijen Lucio A. A.,
Hajra Amiya K.,
Agranoff Bernard W.
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb05446.x
Subject(s) - phosphatidate , phosphatidylinositol , diacylglycerol kinase , chemistry , biochemistry , stimulation , biophysics , phosphatidic acid , free nerve ending , membrane , protein kinase c , neuroscience , anatomy , phospholipid , biology , signal transduction
Carbamylcholine enhances the labeling of phosphatidate and phosphatidylinositol from 32 P i in nerve endings. Approximately 74% of labeled phosphatidate and 85% of labeled phosphatidylinositol produced on muscarinic stimulation are accounted for by tetraenoic species, as detected by argentation TLC. Incubation of membranes derived from nerve endings with [γ‐ 32 P]ATP under conditions of phosphodiesteratic degradation of endogenous polyphosphoinositides resulted in increased labeling of phosphatidate. Approximately 78% of the newly formed phosphatidate was in a tetraenoic fraction. It is concluded that in muscarinically stimulated nerve endings, the diacylglycerol moiety is conserved following diacylglycerol release from polyphosphoinositides through its resynthesis to inositol lipid via phosphatidate.