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Changes of Enzymes Involved in Prostaglandin Metabolism and Prostaglandin Binding Proteins in Rat Brain During Development and Aging
Author(s) -
Ueno Ryuji,
Osama Hiroyoshi,
Urade Yoshihiro,
Hayaishi Osamu
Publication year - 1985
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1985.tb04014.x
Subject(s) - medicine , prostaglandin , endocrinology , gestation , prostaglandin e , metabolism , enzyme , prostaglandin e2 , biology , gestational age , dehydrogenase , chemistry , biochemistry , pregnancy , genetics
In the developing rat brain, the enzymatic formation of prostaglandin D 2 from prostaglandin H 2 increased 60‐fold from day 12 of gestation to birth. The activity still rose gradually to the highest level (90 nmol/min/g wet tissue) at day 7 after birth. The activities of prostaglandin E 2 and F 2α synthetases in rat brain were highest at gestational age 19 days (30 nmol/min/g wet tissue) and at gestational age 14 days (15 nmol/min/g wet tissue), respectively. The specific activity of NADP‐dependent 15‐hydroxy‐prostaglandin D 2 dehydrogenase in rat brain was highest at the earliest gestational age we examined (day 12 of gestation), The specific bindings of prostaglandin D 2 and E 2 to the crude mitochondrial fraction of rat brain were observed from day 16 of gestation and increased to day 7 after birth. Although the activities of the enzymes responsible for prostaglandin metabolism were unchanged postmaturationally, the maximal concentrations of the binding sites on the synaptic membrane for both prostaglandins D 2 and E 2 decreased with constant affinity to less than one‐sixth with age from 1 week to 24 months after birth. These results indicate that prostaglandins may play important roles during maturation and aging in rat brain.