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Biosynthesis of Triglyceride and Other Fatty Acyl Esters by Developing Rat Brain
Author(s) -
Bishop James E.,
Hajra Amiya K.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb12842.x
Subject(s) - diglyceride , acylation , chemistry , triglyceride , biochemistry , biosynthesis , acyl coa , coenzyme a , enzyme , microsome , fatty acid , acyltransferases , sterol o acyltransferase , cholesterol , catalysis , reductase , lipoprotein
The biosynthesis of triglyceride from 1,2‐diglyceride and long‐chain acyl coenzyme A (CoA) was studied in developing rat brain. Diglyceride acyltransferase activity was highest in a microsomal fraction, had a neutral pH optimum, and was stimulated by MgCl 2 . Palmitoyl CoA and oleoyl CoA served equally well as acyl donors. The enzyme catalyzed the acylation of both endogenous diglyceride and several naturally occurring and synthetic exogenous diglycerides. In addition, short‐chain primary and secondary alcohols were found to be acylated under these conditions. A second acylation system, active at low pH, was found to catalyze esterification of ethanol and cholesterol, but not diglyceride, with free fatty acid. These results demonstrate that brain has the capacity to acylate a wide variety of physiological and nonphysiological hydroxyl compounds.