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Developmental Differences Between Soluble and Membrane‐Bound Fractions of Choline‐ O ‐Acetyltransferase in Neonatal Mouse Brain
Author(s) -
Benishin Christina G.,
Carroll Paul T.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb12815.x
Subject(s) - choline acetyltransferase , choline , chemistry , biology , biochemistry , neuroscience , central nervous system
Three fractions (one soluble and two membrane‐bound) of choline acetyltransferase (ChAT) isolated from a nerve ending fraction of mouse forebrain, which have previously been reported to differ in several biochemical and physical aspects, were also found to differ in their rates of postnatal development. At 2 days of age, the activity in all three fractions was very low. Sodium phosphate buffer‐soluble (cytoplasmic) ChAT activity increased significantly by 8 days of age, whereas the ChAT activity of the two membrane‐bound fractions (NaCl‐ and Triton‐soluble) did not increase until 13 days of age. These results suggested that the differences observed between the three fractions of ChAT prepared from mouse brain are not solely artifacts of the isolation procedure.