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Angiotensin‐Converting Enzyme Activity Is Reduced in Brain Microvessels of Spontaneously Hypertensive Rats
Author(s) -
Kobayashi Hideyuki,
Take Keiko,
Wada Akihiko,
Izumi Futoshi,
Magi Maria Sandra
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb12756.x
Subject(s) - medicine , angiotensin converting enzyme , renin–angiotensin system , spontaneously hypertensive rat , angiotensin ii , enzyme , endocrinology , chemistry , blood pressure , biochemistry
Angiotensin‐converting enzyme (ACE) activity in brain microvessels of spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) controls was measured. Cerebral microvessels, prepared from the cerebral cortices by the albumin flotation and glass bead filtration technique, were free of neuronal and glial elements. ACE activity in brain microvessels of SHR was lower than that of WKY. A Woolf‐Augustinsson‐Hofstee plot showed that the reduction of the enzyme activity in SHR was due to a 30% decrease in V max without any change in K m for substrate. The decrease of ACE activity in brain micro‐vessels of SHR may indicate an impairment of the central renin‐angiotensin system and may be related to cerebral microvascular dysfunctions occurring in hypertension.