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Effect of Oxygen on the Induction of Tryptophan Hydroxylase by Adrenergic Agents in Organ Cultures of Rat Pineal Glands
Author(s) -
Sitaram B. R.,
Lees G. J.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb12728.x
Subject(s) - cycloheximide , tryptophan hydroxylase , puromycin , serotonin , endocrinology , tryptophan , medicine , pineal gland , organ culture , adrenergic , biology , enzyme , chemistry , melatonin , biochemistry , protein biosynthesis , amino acid , receptor , in vitro , serotonergic
Rat pineal glands, cultured under 95% air and 5% CO 2 , lost 50% of their tryptophan hydroxylase activity within 5 h. This loss was accelerated by the addition of cycloheximide or puromycin to the medium. Activity was, however, largely maintained in 95% O 2 and 5% CO 2 . Under these conditions, L‐noradrenaline (100 μ.M), L‐iso‐proterenol (10 μM), and dibutyryl cyclic AMP (1 mM) induced enzyme activity. They failed to do so when 95% air and 5% CO 2 was used. Noradrenaline induced serotonin N‐acetyltransferase activity with either atmosphere.