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Activation of Choline Acetyltransferase by Vasoactive Intestinal Peptide
Author(s) -
Luine Victoria N.,
Rostene William,
Rhodes Joseph,
McEwen Bruce S.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb12721.x
Subject(s) - vasoactive intestinal peptide , choline acetyltransferase , endocrinology , medicine , choline , hypothalamus , acetylcholine , neuropeptide , acetylcholinesterase , chemistry , cholinergic , biology , enzyme , biochemistry , receptor
Addition of vasoactive intestinal peptide (VIP) to brain homogenates increased the activity of choline acetyltransferase (ChAT) but not that of acetylcholinesterase or glucose‐6‐phosphate dehydrogenase. Activity of ChAT was increased in the anterior hypothalamus and in the dorsal and ventral hippocampus, but not in the parietal cortex or posterior hypothalamus. Increased activity occurred rapidly after VIP addition to homogenates and was maximal at 10 ‐7 M concentration. Kinetic analysis indicates that the V max of the enzyme is increased and the K m for choline, but not acetyl‐coenzyme A, is decreased in the presence of VIP. Results support a possible VIP‐cholinergic interaction in the CNS.