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Metabolism of 15 NH 3 in Organotypic Cerebellar Explants and Cultured Astrocytes: Studies with Gas Chromatography‐Mass Spectrometry
Author(s) -
Yudkoff Marc,
Nissim Itzhak,
Kim Seung U.,
Pleasure David,
Segal Stanton
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb09731.x
Subject(s) - glutamine , explant culture , glycine , astrocyte , metabolism , chemistry , mass spectrometry , glutamate receptor , alanine , cerebellum , biochemistry , chromatography , biology , amino acid , central nervous system , in vitro , endocrinology , receptor
Gas chromatography‐mass spectrometry was used to study the metabolism of 15 NH 3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady‐state level of 15 NH 3 was present by 1 min in both systems. Steady‐state labeling in l‐[amide‐ 15 N] glutamine, l‐[ 15 N]alanine, l‐[ 15 N]glutamate, and l‐[ 15 N]aspartate was attained by 1 min after 15 NH 3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15 N labeling was noted in either glycine or serine in either system.