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Modulation by Unsaturated Fatty Acids of Norepinephrine‐ and Adenosine‐Induced Formation of Cyclic AMP in Brain Slices
Author(s) -
Baba Akemichi,
Tatsuno Tohru,
Iwata Heitaroh
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb09716.x
Subject(s) - linoleic acid , adenosine , medicine , arachidonic acid , veratridine , chemistry , adenosine deaminase , endocrinology , norepinephrine , fatty acid , biochemistry , biology , enzyme , dopamine , sodium , organic chemistry , sodium channel
The effect of linoleic acid on the formation of cyclic AMP in the slices of guinea pig cerebral cortex was examined. Treatment of the slices with linoleic acid resulted in an increase of basal and of norepinephrine‐stimulated formation of cyclic AMP. The stimulatory effect on the basal level of cyclic AMP was not specific for linoleic acid: the potency of the fatty acid was related to the magnitude of unsaturation. In contrast, the enhancement of norepinephrine‐stimulated formation of cyclic AMP seemed relatively specific for linoleic acid and arachidonic acid. Linoleic acid markedly enhanced the stimulated formation of cyclic AMP by histamine and adenosine, as well that by norepinephrine, without affecting that by excitatory amino acids and veratridine. Theophylline, adenosine deaminase, and 2′‐deoxyadenosine antagonized the effect of linoleic acid. Linoleic acid enhanced the maximum responses to norepinephrine and adenosine without altering the ED 50 values for these agonists. When linoleic acid‐treated slices were washed with Krebs‐Ringer containing defatted bovine serum albumin, both enhancement of the response to norepinephrine and the amount of [ 14 C]linoleic acid incorporated in a free form significantly diminished.