Premium
Regulation of Nervous System‐Specific S‐100 Protein and Enolase Levels in Adipose Tissue by Catecholamines
Author(s) -
Suzuki Fujiko,
Kato Kanefusa,
Nakajima Takashi
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb09708.x
Subject(s) - enolase , adipose tissue , medicine , endocrinology , epinephrine , protein subunit , norepinephrine , catecholamine , chemistry , in vivo , biology , dopamine , biochemistry , immunohistochemistry , microbiology and biotechnology , gene
The effect of catecholamines on the levels of S‐100 protein and nervous system‐specific enolase (NSE) in epididymal adipose tissue of Wistar rats in vivo was examined by sensitive enzyme immunoassay methods. Soluble S‐100 protein levels in the adipose tissue of 9‐12‐week‐old rats (1.46 ± 0.19 μg/mg protein) were decreased to <50% of those of controls by serial injection (for 4–7 days) of epinephrine (0.1 mg/day) or norepinephrine (0.15 mg) with, however, little effect on the levels of membrane‐bound (pentanol‐extractable) S‐100 protein. A significant decrease in the soluble S‐100 protein levels was observed at 2 h after a single injection of epinephrine (1.04 ± 0.13 μg/mg protein). On the other hand, levels of NSE subunit (γ subunit or 14–3–2 protein) in adipose tissue (0.51 ± 0.03 γγ‐equivalent pmol/mg protein) were increased to 170% of control by serial injection (for 7 days) of epinephrine or norepinephrine with little change of the level of enolase a subunit on a mg protein basis. Isoproterenol had no apparent effect on the levels of soluble S‐100 protein and NSE subunit. These results suggest that the levels of S‐100 protein and NSE in adipose tissue are regulated by catecholamines.