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Similarities and Differences Between High‐Affinity Binding Sites for Cocaine and Imipramine in Mouse Cerebral Cortex
Author(s) -
Reith Maarten E. A.,
Allen Donald L.,
Sershen Henry,
Lajtha Abel
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06703.x
Subject(s) - imipramine , cerebral cortex , binding site , chemistry , cortex (anatomy) , biochemistry , pharmacology , biology , neuroscience , medicine , alternative medicine , pathology
Previously we found close similarities between high‐affinity binding sites for [ 3 H]cocaine and those for [ 3 H]imipramine in the mouse cerebral cortex in regard to their association with neuronal uptake of serotonin. In the present study we investigated whether the two ligands bind to the same site. The two ligands had the following high‐affinity binding properties in common: localization in both synaptosomal and microsomal fractions; vulnerability to treatment with N ‐ethylmaleimide, trypsin, and phospholipase A 2 ; and resistance to exposure to dithiothreitol. In contrast, cocaine binding in the cerebral cortex was more sensitive to heat inactivation than imipraminebinding. In addition, the mechanism by which cocaine inhibited [ 3 H]imipramine binding differed from that by which imipramine inhibited [ 3 H]cocaine binding. These data suggest that the high‐affinity binding sites for [ 3 H]cocaine and [ 3 H]imipramine in the cerebral cortex are distinct entities.