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Comparison of Two Superfusion Systems for Study of Neurotransmitter Release from Rat Cerebral Cortex Slices
Author(s) -
Korpi E. R.,
Oja S. S.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06701.x
Subject(s) - veratridine , depolarization , cerebral cortex , neurotransmitter , neuroscience , chemistry , calcium , cortex (anatomy) , biophysics , biology , central nervous system , sodium , organic chemistry , sodium channel
Abstract: Depolarization‐induced release of [ 3 H]γ‐aminobutyric acid (GABA) and [ 3 H]noradrenaline (NA) from rat cerebral cortex slices was studied in two superfusion systems: one with stationary and the other one with continuously shaken slice compartments. Calcium‐dependent depolarization‐induced release of GABA and NA could be demonstrated only with shaken slices. GABA, but not NA, could also be released by high K + media and veratridine from stationary slices. Synaptic transmitterreleasing mechanisms are apparently damaged in stationary slices, possibly due to impaired energy metabolism.