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Interaction of Neuropeptides and Cultured Glial (Müller) Cells of the Chick Retina: Elevation of Intracellular Cyclic AMP by Vasoactive Intestinal Peptide and Glucagon
Author(s) -
Koh ShayWhey M.,
Kyritsis A.,
Chader Gerald J.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06697.x
Subject(s) - vasoactive intestinal peptide , neuropeptide , intracellular , medicine , retina , endocrinology , glucagon , biology , chemistry , peptide , microbiology and biotechnology , neuroscience , biochemistry , hormone , receptor
Vasoactive intestinal peptide (VIP) and, to a lesser extent, glucagon were found to increase intraclular cyclic AMP rapidly in cultured glial (Müller) cells of the chick embryo retina. Although VIP elicited higher cyclic AMP accumulation than glucagon at each concentration tested, the half‐maximal concentrations were similar, i.e., 6 × 10 −8 M for VIP and 8 × 10 −8 M for glucagon. Secretin had a minimal effect on cyclic AMP accumulation even at a very high (5 × 10 −6 M ) concentration. Several other peptide and nonpeptide putative agonists also had little effect on cyclic AMP accumulation. The cultured Müller cell may thus be a useful model for examining VIP and glucagon effects on glial elements of the CNS.