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The Effects of Detergents on the Composition of Postsynaptic Densities
Author(s) -
Somerville Robert A.,
Merz Patricia A.,
Carp Richard I.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06695.x
Subject(s) - postsynaptic potential , chemistry , composition (language) , solubilization , tubulin , biophysics , extraction (chemistry) , intermolecular force , chromatography , size exclusion chromatography , sodium , biochemistry , microtubule , organic chemistry , molecule , biology , enzyme , microbiology and biotechnology , philosophy , linguistics , receptor
A method of purifying postsynaptic densities (PSD) of Cohen et al. (1977) has been modified, primarily by the substitution of octyl glucoside as the detergent used to solubilize synaptosomal fractions. Subsequent extraction with other detergents resulted in the selective removal of specific polypeptides. In particular sulphobetaine 3–14 removed most of the β‐tubulin but not α‐tu‐ bulin. Sodium N ‐ lauroyI sarcosinate completely destroyed the structural integrity of the PSD when the invitro formation of intermolecular disulphide bonds was minimized. These results suggest that the structure of PSDs is more labile than previously thought and demonstrate a technique for further examining their composition.