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The Relationship Between Arachidonic Acid Release and Catecholamine Secretion from Cultured Bovine Adrenal Chromaffin Cells
Author(s) -
Frye Roy A.,
Holz Ronald W.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06690.x
Subject(s) - arachidonic acid , chromaffin cell , catecholamine , endocrinology , medicine , adrenal medulla , secretion , biology , chemistry , biochemistry , enzyme
Increased arachidonic acid release occurred during activation of catecholamine secretion from cul‐ tured bovine adrenal medullary chromaffin cells. The nicotinic agonist l,l‐dimethyl‐4‐phenylpiperazinium (DMPP) caused an increased release of prcincubated [ 3 H]arachidonic acid over a time course which corre‐ sponded to the stimulation of catecholamine secretion. Like catecholamine secretion, the DMPP‐induced [ 3 H]arachidonic acid release was calcium‐dependent and was blocked by the nicotinic antagonist mecamylamine. Depolarization by elevated K + , which induced catechol amine secretion, also stimulated arachidonic acid release. Because arachidonic acid release from cells probably re‐ sults from phospholipase A 2 activity, our findings indicate that phospholipase A 2 may be activated in chromaffin cells during secretion.