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Regulation of Cyclic AMP Accumulation by Peptide Hormone Receptors in Immunocytochemically: Defined Astroglial Cells
Author(s) -
Evans Tony,
McCarthy Ken D.,
Harden T. Kendall
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06688.x
Subject(s) - receptor , medicine , endocrinology , vasoactive intestinal peptide , bombesin , somatostatin , biology , agonist , neurotensin , somatostatin receptor , neuropeptide , chemistry
Primary cultures of neonatal murine brain have been reported to express multiple receptors that regulate adenylate cyclase activity. Since for the most part these results were obtained with mixed cell cultures, it has been difficult to define receptor profiles for specific cell types. With this concern in mind a series of studies has been initiated designed to identify specific receptors present on highly purified, immunocytochemically defined astroglia derived from the cerebral cortices of neonatal rats. In this study the capacity of a variety of peptide hormones to regulate cyclic AMP metabolism in these cells was examined. Fibroblasts derived from the meninges represent a predictable source of contamination in primary CNS culture. Thus, to assign more clearly specific receptors to the astroglial cell population, receptor‐mediated regulation of cyclic AMP accumulation was also examined in fibroblasts. Cyclic AMP accumulation in astroglia was stimulated by catecholamines (acting at β 1 ,‐ adrenergic receptors), prostaglandin E 1 , vasoactive intestinal polypeptide, α‐melanocyte‐stimulating hormone, and adrenocorticotropin. Bombesin, luteinizing hormonereleasing hormone, neurotensin, thyrotropin‐releasing hormone, somatostatin, secretin, and vasopressin did not significantly increase cyclic AMP levels in these cultures. Catecholamines, acting at α 2 ‐adrenergic receptors, and somatostatin inhibited agonist‐stimulated cyclic AMP ac cumulation. In meningeal cell cultures catecholamines (acting at β 2 ‐ and α 2 ‐adrenergic receptors) and prostaglandin E 1 , regulated cyclic AMP levels. However, vasoactive intestinal peptide did not stimulate and somatostatin did not inhibit cyclic AMP accumulation in these cells.