Premium
Sequence of Guinea Pig Myelin Basic Protein
Author(s) -
Deibler Gladys E.,
Martenson Russell E.,
Krutzsch Henry C.,
Kies Marian W.
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06683.x
Subject(s) - guinea pig , peptide , peptide sequence , sequence (biology) , amino acid , myelin basic protein , biochemistry , biology , chemistry , microbiology and biotechnology , myelin , genetics , gene , neuroscience , central nervous system
This paper proposes a tentative amino acid sequence of guinea pig myelin basic protein obtained by comparison of peptide fragments of the guinea pig and bovine proteins. Analyses of the tryptic peptides confirmed the known sequence differences in the NH 2 ‐terminal half of the molecule and showed that in the COOH‐terminal half of the guinea pig protein Ser 131 was missing, Ala 136 ‐His 137 was deleted, Leu 140 was replaced by the, and an extra Ala was inserted somewhere within sequence 142–151 (tryptic peptide T23). Sequence determination of guinea pig tryptic peptides corresponding to residues 130–134 (T20), 135–138 (T21), and 142–151 (T23) of the bovine protein confirmed the above sequence changes and placed the extra Ala between Gly 142 and His 143 . The sequence of the region corresponding to bovine residues 130–143 is thus Ala‐Asp‐Tyr‐Lys‐Ser‐Lys‐Gly‐Phe‐Lys‐Gly‐Ala‐His. No species differences were observed in the amino acid compositions of the remaining tryptic peptides obtained from the COOH‐terminal half of the molecule. Based upon these results, the guinea pig basic protein contains 167 amino acid residues and has a molecular weight of 18,256.