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Regional Distribution of Prostaglandins D 2 , E 2 , and F 2α and Related Enzymes in Postmortem Human Brain
Author(s) -
Ogorochi Toshiya,
Narumiya Shuh,
Mizuno Noboru,
Yamashita Kouwa,
Miyazaki Hiroshi,
Hayaishi Osamu
Publication year - 1984
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1984.tb06680.x
Subject(s) - prostaglandin , cerebrum , medicine , endocrinology , hypothalamus , radioimmunoassay , enzyme , prostaglandin e2 , chemistry , prostaglandin e , prostaglandin d2 , dehydrogenase , biology , biochemistry , central nervous system
The presence of prostaglandins D 2 , E 2 , and F 2α was demonstrated and their contents measured in various regions of postmortem human brain, pineal body, and pituitary by using specific radioimmunoassays and gas chromatography‐mass spectrometry. The three prostaglandins were widely distributed in similar concentrations ranging from several hundred pg/g wet weight to about 40 ng/g wet weight. Prostaglandins D 2 and E 2 showed consistent and similar regional distributions in all six brains tested; amounts were high in pineal body, pituitary, olfactory bulb, and hypothalamus. On the other hand, prostaglandin F 2α was distributed more evenly. Prosta‐ glandin D synthetase and prostaglandin E synthetase activities were found in cerebrum homogenate from a single subject and were recovered from the 100,000 × g supernatant. The presence of 1 m M glutathione, reduced form, markedly stimulated the activity of prostaglandin E synthetase, but did not affect prostaglandin D synthetase activity. Activity of 15‐hydroxyprostaglandin dehydrogenase was found in the cerebrum homogenate and was partially purified. This enzyme required NADP as a cofactor and copurified with prostaglandin E 9‐ketoreductase.